Facts-enriched characterization of mobile populations. Morphological facts from pictures can add to the richness of flow cytometry knowledge. Such as, the figure exhibits an if not regular apoptosis assay employing Annexin V and PI, adding cell imaging to characterize cells in each populace to reveal morphologically distinct functions. These insights could not happen to be received from multiplex staining on your own.
Regardless of whether you’re a rookie or a specialist, examine our resources to progress your analysis and know-how in flow cytometry.
Merge acoustic concentrating technology with superior-parameter Assessment for versatile spectral unmixing and conventional workflows.
Discover new insights into your experimental details by visualizing populations making use of put together fluorescence and morphology facts. Our computer software interface wizards help downsampling, concatenation (merged data files) and dimensionality instruments which might be constructed in the computer software for an intuitive, streamlined facts Examination expertise.
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Below, a highly trained user has gated singlets confidently. Just after evaluating the guide singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate has a lot more than four% aggregates.
Optimizing gating system. Even sturdy manual singlet gating is error-vulnerable and stays a subjective conclusion point in Pretty much all flow cytometry assays. Imaging can be used to confirm and adjust gates to include only one cells of interest.
Steady graphic high quality even at higher throughput Acoustic focusing and a high-pace digital camera Blend to picture these T cells consistently at very low or large flow rates. Conveniently adjust focus and digicam options to fulfill experimental needs.
The Attune NxT cytometer may be the trusted workhorse instrument your lab can rely upon for regular and trustworthy overall performance. The Attune CytPix design includes a high-velocity brightfield digicam and AI-pushed graphic analysis software program, with latest updates together with device Understanding equipment and an intuitive person interface, enabling enhanced data precision, accuracy, and quicker insights into sample biology.
Perhaps most importantly, these activities contain cells of Evidently different phenotypes which could lead to incorrect conclusions with regards to double optimistic situations (especially in exceptional populations).
AI-pushed automated impression Evaluation While using the Attune Cytometric Program interprets celebration functions into distinct morphology parameters which might be coupled with regular fluorescence and scatter parameters to reveal extremely reproducible population adjustments and special subsets.
We previously shown the power of imaging CAR-T/Ramos mobile interactions. Let’s evaluate just the population of greatest fascination, the double constructive gatherings, To find out more. We can now use prolonged morphological parameters (below, circularity and skewness of depth) to further examine the functions of those populations and refine gating on these events, expanding information robustness.
Leverage the power of Highly developed technologies and innovation to accelerate your discoveries. Rely on inside the Attune Flow Cytometer spouse and children to deliver the trustworthiness, velocity, click here and precision that established your work apart.
No wash/no lyse leukocyte Investigation with imaging. Cells had been obtained from 24-hour-old blood by dilution in one mM EDTA ( Specialist UX/UI permits users to coach AI types for expanded applications. (A) The “Practice Design” aspect offers annotation resources and serious-time model education accuracy.
At the guts of each Attune Flow Cytometer lies our revolutionary acoustic focusing technologies, which utilizes audio waves to meticulously align cells within the flow cell. This impressive strategy surpasses traditional hydrodynamic methods, leading to faster processing speeds, Remarkable information high quality, and amplified sample throughput.
The Attune Xenith cytometer is really a high-parameter spectral analyzer, supplying each spectral unmixing and conventional flow abilities for extensive and adaptable cell Evaluation.
The Attune CytPix Flow Cytometer makes it possible for users to visualise samples throughout acquisition, confirm the accuracy of gating techniques, and help results with responsible documentation for publication functions.
What seemed to be a homogeneous population is definitely far more numerous—an insight that should be thought of when interpreting benefits.