Details-enriched characterization of cell populations. Morphological information and facts from photographs can incorporate to your richness of flow cytometry information. Such as, the figure demonstrates an otherwise regular apoptosis assay utilizing Annexin V and PI, introducing mobile imaging to characterize cells in Just about every population to reveal morphologically unique characteristics. These insights could not are actually received from multiplex staining by itself.
As samples are acquired around the Attune CytPix Flow Cytometer, the superior-pace brightfield camera captures and stores images of detected activities in a charge approximately 6,000 photographs for every 2nd, based on the flow fee and picture dimension. For larger versatility, Attune Cytometric Software permits you to change the impression seize frequency as wanted and select a specific gate for graphic seize, if required.
Blend acoustic concentrating technological know-how with higher-parameter analysis for versatile spectral unmixing and conventional workflows.
Find new insights into your experimental info by visualizing populations making use of mixed fluorescence and morphology info. Our software package interface wizards enable downsampling, concatenation (merged information) and dimensionality equipment which have been created into the computer software for an intuitive, streamlined details Examination knowledge.
"A picture is just not fairly value one thousand dots, nevertheless it really helps you to see pictures. It is excellent to check out illustrations or photos that correlate with staining. For instance, AnnexinV-constructive cells which might be morphologically different than healthful cells, or CD14+ monocytes which can be more substantial and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
In addition, it can be illuminating to view what number of doublets slip via common singlet gates, and good to confirm the status of sudden double-optimistic events in my Evaluation."
Pair your Attune flow cytometer with the autosampler for increased workflow efficiency, and incorporate robotic integration for optimum throughput.
Dependable graphic top quality even at superior throughput Acoustic focusing in addition to a higher-speed digicam Mix to picture these T cells constantly at reduced or superior flow premiums. Effortlessly adjust aim and digicam configurations to meet experimental prerequisites.
Early indications of this contamination experienced in the beginning been dismissed as particles. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also helps you to use morphological attributes to find out appealing subpopulations that would not be apparent from flow cytometry information by itself.
Most likely most of all, these activities incorporate cells of Evidently different phenotypes which could lead on to incorrect conclusions regarding double positive activities (especially in uncommon populations).
Seem waves empower exact mobile alignment, resulting in Extraordinary info high quality and ~10X a lot quicker sample flow fees.
We Formerly shown the strength of imaging CAR-T/Ramos mobile interactions. Allow’s take a look at just the populace of biggest desire, the double good functions, to learn more. We could now use prolonged morphological parameters (here, circularity and skewness of intensity) to even more analyze the attributes of such populations here and refine gating on these functions, escalating data robustness.
Designed-in dimensionality reduction applications greater resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to substantiate predicted population resolution in lysed full human blood. User can look at events from Each and every cluster for visual affirmation of cell phenotypes.
Adaptable payment prepare from Agilent Fiscal Remedies permit you to have the crucial instrumentation you will need for fast undertaking, or to increase your lab.
Gating on these extended parameters permits you to promptly and accurately establish populations of desire and confirm gating approach with little if any handbook assessment. The Attune software offers entry to state-of-the-art equipment Discovering Examination tools in just an expertly designed consumer interface, rendering it extremely simple to operate.
The Attune Xenith cytometer is a superior-parameter spectral analyzer, providing both equally spectral unmixing and conventional flow capabilities for extensive and adaptable mobile Examination.
The Attune CytPix Flow Cytometer enables users to visualise samples throughout acquisition, confirm the accuracy of gating techniques, and assistance benefits with reputable documentation for publication uses.
What appeared to be a homogeneous populace is actually extra various—an Perception that needs to be regarded when interpreting outcomes.